Earth System Science Data
Earth System Science Data
Earth System Science Data
Articles | Volume 17, issue 2
Articles | Volume 17, issue 2
https://doi.org/10.5194/essd-17-393-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/essd-17-393-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 17, issue 2
Data description paper
 | 
04 Feb 2025
Data description paper |  | 04 Feb 2025

Global biogeography of N2-fixing microbes: nifH amplicon database and analytics workflow

Michael Morando, Jonathan D. Magasin, Shunyan Cheung, Matthew M. Mills, Jonathan P. Zehr, and Kendra A. Turk-Kubo

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Latest update: 28 Oct 2025
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Short summary
Nitrogen is crucial in ocean food webs, but only some microbes can fix N2 gas into a bioavailable form. Most are known only by their nifH gene sequence. We created a software workflow for nifH data and ran it on 944 ocean samples, producing a database (DB) that captures the global diversity of N2-fixing marine microbes and the environmental factors that influence them. The workflow and DB can standardize analyses of past and future nifH datasets to enable insights into marine communities.
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