Global biogeography of N<sub>2</sub>-fixing microbes: <i>nifH</i> amplicon database and analytics workflow

Morando, Michael; Magasin, Jonathan D.; Cheung, Shunyan; Mills, Matthew M.; Zehr, Jonathan P.; Turk-Kubo, Kendra A.

doi:https://doi.org/10.5194/essd-17-393-2025

Articles | Volume 17, issue 2

https://doi.org/10.5194/essd-17-393-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/essd-17-393-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 17, issue 2

Data description paper

|

04 Feb 2025

Data description paper |

| 04 Feb 2025

Global biogeography of N₂-fixing microbes: nifH amplicon database and analytics workflow

Michael Morando, Jonathan D. Magasin, Shunyan Cheung, Matthew M. Mills, Jonathan P. Zehr, and Kendra A. Turk-Kubo

Supplement

https://doi.org/10.5194/essd-17-393-2025-supplement

Data sets

nifH ASV database [Global biogeography of N2-fixing microbes: nifH amplicon database and analytics workflow] Michael Morando et al. https://doi.org/10.6084/m9.figshare.23795943.v2

Model code and software

DADA2 nifH pipeline Michael Morando et al. https://github.com/jdmagasin/nifH_amplicons_DADA2

nifH ASV workflow (post-pipeline stages) Michael Morando et al. https://github.com/jdmagasin/nifH-ASV-workflow

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Short summary

Nitrogen is crucial in ocean food webs, but only some microbes can fix N₂ gas into a bioavailable form. Most are known only by their nifH gene sequence. We created a software workflow for nifH data and ran it on 944 ocean samples, producing a database (DB) that captures the global diversity of N₂-fixing marine microbes and the environmental factors that influence them. The workflow and DB can standardize analyses of past and future nifH datasets to enable insights into marine communities.