Sampling and analysis methodology and spatial radionuclide deposition data from the 60 km area around the Chernobyl Nuclear Power Plant, sampled in 1987 by the Ukrainian Institute of Agricultural Radiology, are useful for reconstructing doses to human and wildlife populations, answering the current lack of scientific consensus on the effects of radiation on wildlife in the Chernobyl Exclusion Zone and evaluating future management options for the Chernobyl-impacted areas of Ukraine and Belarus.