Multiple well fields in Waterloo Region, ON, Canada, draw water that enters the groundwater system from rainfall and snowmelt within the Alder Creek watershed. The rates of recharge of the underground aquifers and human impacts on streamflow are important issues that are typically addressed using computer models. Field observations such as groundwater and stream levels were collected between 2013 and 2018 to provide data for models. The data are available at https://doi.org/10.20383/101.0178