10 May 2021

10 May 2021

Review status: a revised version of this preprint was accepted for the journal ESSD.

An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth

Elianne Egge1,a, Stephanie Elferink2, Daniel Vaulot3,4, Uwe John2, Gunnar Bratbak5, Aud Larsen6, and Bente Edvardsen1 Elianne Egge et al.
  • 1University of Oslo, Department of Biosciences, Section for Aquatic Biology and Toxicology, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY
  • 2Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung. Am Handelshafen 12, Bremerhaven DE-27570, GERMANY
  • 3UMR7144, CNRS, Sorbonne Université, Station Biologique de Roscoff. Place Georges Teissier, FR-29682 Roscoff, FRANCE
  • 4Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 SINGAPORE
  • 5University of Bergen, Department of Biological Sciences, PO Box 7803, NO-5020 Bergen, NORWAY
  • 6NORCE Norwegian Research Centre, PO Box 7810, NO-5020 Bergen, NORWAY
  • apresent address: University of Duisburg-Essen, Fakultät für Biologie, Universitätsstr. 5, DE-45141 Essen, GERMANY

Abstract. Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth) and mesopelagic zones (200–1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45–200 μm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Altogether 6,536 protist ASVs were obtained (including 54 fungal ASVs). Both ASV richness and taxonomic composition were strongly dependent on size-fraction, season, and depth. ASV richness was generally higher in the smaller fractions, and higher in winter and the mesopelagic samples than in samples from the well-lit epipelagic zone during summer. During spring and summer, the phytoplankton groups diatoms, chlorophytes and haptophytes dominated in the epipelagic zone. Parasitic and heterotrophic groups such as Syndiniales and certain dinoflagellates dominated in the mesopelagic zone all year, as well as in the epipelagic zone during the winter. The dataset is available at (Egge et al. 2014).

Elianne Egge et al.

Status: final response (author comments only)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on essd-2021-133', Anonymous Referee #1, 09 Jun 2021
    • AC1: 'Reply on RC1', Elianne Egge, 03 Jul 2021
  • RC2: 'Comment on essd-2021-133', Anonymous Referee #2, 14 Jul 2021

Elianne Egge et al.

Data sets

An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth Egge, Elianne; Elferink, Stephanie; Vaulot, Daniel; John, Uwe; Bratbak, Gunnar; Larsen, Aud; Edvardsen, Bente

Elianne Egge et al.


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Short summary
Here we present a data set of DNA-sequences obtained from size-fractionated seawater samples from the Arctic Ocean, that are used to identify taxonomic groups of unicellular plankton. This data set can be used to investigate the diversity and distribution of plankton groups both by season and by depth, and thus increase our understanding of the factors influencing the dynamics of this important part of the Arctic marine ecosystem.